Peasy®-blunt zero cloning kit

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August undefined, 2024

WebpEASY-Blunt Zero Cloning Kit. pEASY ® Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed upon plating. Therefore, blue/white selection is not required. Cat. No. CB501. Specification: WebSep 1, 2024 · Removal of the MCS fragment from the pJET1.2M plasmid produces the pJET1.2/blunt cloning vector ready for gene cloning. The self-made pJET1.2/blunt cloning vector exhibited a cloning...

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WebDec 6, 2024 · About 1-cm-long root segments were incubated in 10% KOH solution at 95°C for 100 min, rinsed with distilled water, bleached with 10% hydrogen peroxide solution for 15 min, acidified with 0.2 mol/L hydrochloric acid for 10 min, and stained with 0.05% trypan blue in lactate phenol for 1 min. WebOct 1, 2016 · Features of the pEASY-Blunt Zero Cloning Kit. 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning … edward jones athens georgia

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WebAug 13, 2024 · The amplified products were examined by 1% agarose gel electrophoresis and purified using a gel extraction kit (TransGen Biotech). The purified cDNAs were cloned into pEASY®-Blunt Zero Cloning Vector (TransGen Biotech) and transformed into E. coli Trans1-T1 competent cells (TransGen Biotech). Positive transformants were identified by … WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 WebpEASY® -Blunt Zero Cloning Kit 目录号： CB501-01 单价：￥780 规格： 20 rxns 60 rxns 数量： - + 说明书载体信息质检报告立即下单加入购物车产品详情介绍该载体通过自杀 … edward jones athens ga

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Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II …

WebFeatures of the pEASY®-T5 Zero Cloning Kit. 5 minutes fast ligation of Taq-amplified (3’A overhangs) PCR products. High cloning efficiency. Positive clones up to 100%. No … WebThe Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector … consumer cellular email to sms gatewayWebApr 11, 2024 · The PCR program consisted of 3 min at 95°C, followed by 35 cycles of 15 s at 95°C, 15 s at 55°C, and 120 s at 72°C, followed by a 5 min extension at 72°C. The PCR product was inserted into the pEASY-Blunt Simple Cloning vector (TransGen Biotech, Beijing, China) and then transformed into DH5α for sequencing. consumer cellular factory reset

"WebZero Blunt ® TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K2800-02) is shipped with an ... " - Peasy®-blunt zero cloning kit

Peasy®-blunt zero cloning kit

Analyses of a chromosome-scale genome assembly reveal the …

WebpEASY® -T5 Zero Cloning Kit pEASY® -Blunt Zero Cloning Kit pEASY® -Uni Seamless Cloning and Assembly Kit pEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell WebApr 25, 2024 · The tps7 gene was amplified from cDNA by PCR with primers and cloned into the plasmid pEASY-Blunt, resulting in pEASY-tps7. The tps7 gene and plasmid were amplified from pEASY-tps7 and pET-28a( +), respectively, and the two fragments were ligated using the pEASY-Blunt Zero Cloning Kit (TransGen Biotech, Beijing), resulting in …

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WebOct 8, 2024 · The amplified strips were connected with pEASY-Blunt Zero Cloning Kit after glue recovery, and the ligation products were sent to Sangon Bioengineering (Shanghai) Co., Ltd for sequencing. Whether... WebThe Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector …

WebStrataClone Blunt PCR Cloning Kit 5 Map for the StrataClone Blunt PCR Cloning Vector pSC-B-amp/kan Feature Nucleotide Position β-galactosidase α-fragment coding sequence (lacZ´) 1–354 Multiple cloning site (MCS) 57–197 PCR product insertion site 123 kanamycin resistance ORF 465-1256 ampicillin resistance (bla) ORF 1268–2125 WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: …

WebJul 9, 2024 · （3 ）常用试剂和引物 pEASY-Blunt Zero Cloning Kit 购自于北京全式金生物技术有限公司，植物RNA 小量提取试剂盒购自上海莱枫生物科技有限公司；Pfu DNA 聚合酶、所有限制性内切酶、 RNase A 、Taq DNA 聚合酶、T4 DNA 连接酶、质粒小提试剂盒、DNA 分子量标志、DNA 凝胶 ...

WebpEASY-Blunt (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer

WebBranching is speculated to contribute to the plant architecture and crop yield. As a quantitative trait, branching is regulated by multiple genes in non-heading Chinese cabbage (NHCC). Several related candidate genes have been discovered in previous studies on the branching of NHCC, but their specific functions and regulatory mechanisms still need to … consumer cellular dropped callsWeb5 min TA/Blunt-Zero Cloning Kit C601. USD$ 201.00. ClonExpress II One Step Cloning Kit C112. USD$ 217.00. Ultra-Universal TOPO Cloning Kit C603. USD$ 196.00. Mut Express MultiS Fast Mutagenesis Kit V2 C215 ... edward jones avon indianaWeb一、采购人名称：石河子大学二、供应商名称：石河子市北泉镇诺泰实验用品经销部三、采购项目名称：石河子大学网上超市项目四、采购项目编号： ***** 五、合同编号： 11n ***** 六、合同内容： consumer cellular find my phoneWebPeasy Blunt Cloning Vector, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. stars, based on 1. Price from $ to $1999.99. peasy blunt cloning vector - by Bioz Stars , 2024-02. edward jones attorney fort worthWebpEASY-Blunt Simple (linearized) Sequence and Map Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a … consumer cellular easy smartphoneWeb7 rows · •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable ... consumer cellular family plansWebAug 9, 2024 · The pJET1.2/blunt cloning vector is a component of the CloneJET PCR Cloning Kit (Catalogue number: K1231), and it is not sold individually. It contains an eco47IR gene that encodes for a restriction enzyme Eco 47I that is harmful to the common laboratory E. coli strains used in molecular cloning. edward jones aum 2021